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Engineered Spirulina platensis for treating rheumatoid arthritis and restoring bone homeostasis | Nature Communications
In brief, embryos were seeded onto mouse embryonic fibroblasts (MEFs) and allowed to attach for 3 days in knockout DMEM (Thermo Fisher, 10829018) supplemented with 20% knockout Serum Replacement (Thermo Fisher, 10828028), GlutaMAX (1.7 mM final, Thermo Fisher Scientific, 35050038), 1× non-essential amino acids (Thermo Fisher, 11140-035), 1× β-mercaptoethanol (Thermo Fisher, 21985023), 1× penicillin/streptomycin (Life Technologies, 15140148) and 1,000 U ml −1 LIF
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Jan 2015;20(1):85-93.
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